Data/hora: 20/05/2024 15:06:08

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 18/06/2012
Data da última atualização: | 15/09/2014
Autoria: | CAVALCANTE, A. C. R.; FERREIRA, A. M.; MELO, M. N.; FUX, B.; BRANDÃO, G. P.; VITOR, R. W. A.
Afiliação: | ANTONIO CEZAR ROCHA CAVALCANTE, CNPC; A.M. Ferreira.
Título: | Virulence and molecular characterization of Toxoplasma gondii isolated from goats in Ceará, Brazil.
Ano de publicação: | 2007
Fonte/Imprenta: | Small Ruminant Research, Amsterdam, v. 69, n. 1/3, p. 79-82, May, 2007.
DOI: | 10.1016/j.smallrumres.2005.12.023
Idioma: | Inglês
Conteúdo: | Abstract - One hundred and sixty-nine fragments of heart muscle collected from goats in the State of Cear´ a, Brazil, were analyzed by mouse bioassay with the aim of isolating Toxoplasma gondii.Two T. gondii isolates, named G1 and G2, were obtained and were characterized by PCR-RFLP. In addition, their virulence was evaluated by experimental inoculation into BALB/c mice. The G1 isolate presented high virulence leading to 100% mortality of mice after inoculations with 101, 102, and 103 tachyzoites. The G2 isolate presented low virulence and none of the doses tested lead to mortality of mice. The PCR-RFLP analysis showed that the two isolates are recombinants of the types I/III. However, they differ in the haplotype combination for the genotypes analyzed.
Palavras-Chave: | PCR-RFLP.
Thesagro: | Caprino; Toxoplasma Gondii.
Thesaurus Nal: | Goats; Virulence.
Categoria do assunto: | --
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (25641-1) - UPC - AP - DD -  -  -  - DIS